About
Dr. Niraj Kumar Singh is currently serving as Associate Professor and Associate Dean, School of Forensic Science, at the National Forensic Sciences University (NFSU), Nagpur Campus, India. He also serves as Coordinator and Head of Academics at the campus. With over 20 years of experience in teaching, research, and academic administration, Dr. Singh has made significant contributions to research and higher education. He has successfully completed research projects funded by various agencies, including UGC, SERB-DST, DBT, and GSBTM. He has published more than 35 original research articles in reputed international journals, authored one book, and contributed five book chapters. He has supervised three Ph.D. scholars and guided more than 50 postgraduate students in their dissertation research.
Dr. Singh's primary research interests include molecular biology, bioinformatics, and the investigation of potential inhibitors of beta-secretase 1 (BACE-1), also known as beta-site amyloid precursor protein-cleaving enzyme 1, which plays a critical role in the production of amyloid-beta (Aβ) peptides associated with Alzheimer's disease. His research focuses on evaluating a naturally occurring compound, C-PBP (PE), as a potential BACE-1 inhibitor. Bioinformatics analyses and biophysical investigations have indicated promising inhibitory potential of PE against BACE-1. The binding interactions are characterised by determining association and dissociation rate constants and binding thermodynamics using surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC), respectively. The effects of PE on BACE-1 enzymatic activity are further evaluated using a synthetic decapeptide substrate to determine relevant kinetic parameters.
Additionally, his research incorporates in vivo validation approaches to investigate the potential effects of PE on amyloid-beta aggregation, employing Thioflavin T staining in a mutant Caenorhabditis elegans model (CL4176). His laboratory integrates molecular biology, bioinformatics, biophysical characterisation, and experimental validation to investigate biologically relevant molecular interactions and potential therapeutic strategies for neurodegenerative diseases.
No. of Publications
Research Paper
35
Book
1
Book Chapter
